TRI Reagent® RT is an upgraded version of the single-step method of RNA isolation. TRI Reagent® RT is based on the phase separation method using phenol and guanidine thiocyanate. This patent-pending reagent substantially improves the quality of isolated RNA. Unlike the previous single-step methods, TRI Reagent® RT isolates RNA that is free of DNA contamination. The purity of RNA was tested by a 35-cycle PCR. Thus, no DNase treatment is necessary to use the isolated RNA in RT-PCR. Also, the isolated RNA solubilizes more quickly and easily as compared with the older single-step methods.
TRI Reagent® RT provides a reliable, cost-effective and efficient method of RNA isolation. It is a comprehensive reagent available for analysis of gene expression. TRI Reagent® RT isolates RNA from a wide variety of samples of human, animal, plant, yeast, bacterial and viral origin. TRI Reagent® RT also can be used to simultaneously isolate RNA, DNA and proteins from the same sample. The RNA isolation is completed in less than 1 hour. In addition to total RNA, TRI Reagent® RT can be used to isolate high and low molecular weight RNA as separate fractions.
TRI Reagent® RT (RT 111)
TRI Reagent® RT is used for RNA isolation from tissues, pelleted cells and cells grown in monolayer. Fifty milliliters is sufficient to process 50 samples, each containing 50 mg of tissue. Expected yields range from 50 – 700 μg of RNA per sample, depending upon the tissue source.
- Chomczynski, P. and Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanate phenol-chloroform extraction. Anal. Biochem. 162:156-159, 1987.
- Chomczynski, P., US patents 4,843,155 and 5,346,994.
- Chomczynski, P. A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. BioTechniques 15:532-537, 1993.