TRI Reagent® is a patented reagent for the isolation of total RNA or for the simultaneous isolation of RNA, DNA and proteins. The reagent is an improved version of the popular single-step method for total RNA isolation (1,2,3). It is a monophase solution containing phenol and guanidine thiocyanate. TRI Reagent® provides a reliable, cost effective and efficient method of RNA isolation. TRI Reagent® allows for a comprehensive analysis of gene expression in a variety of samples of human, animal, plant, yeast, bacterial and viral origin. RNA isolation is complete in less than one hour, and DNA and protein isolations in less than three hours. Three versions of TRI Reagent® are available, each designed for optimal isolation efficiency from certain types of samples.
TRI Reagent® (TR 118)
TRI Reagent® is used for RNA isolation from tissues, pelleted cells and cells grown in monolayer. Fifty milliliters is sufficient to process 50 samples, each containing 50 – 100 mg of tissue. Expected yields range from 50 – 700 μg of RNA per sample, depending upon the tissue source.
US Patents 4,843,155 and 5,346,994.
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- Chomczynski, P. A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. BioTechniques. 15: 532-537. 1993.
- Chomczynski, P., Bowser-Finn, R. and Sabatini, L. A reagent for the single-step isolation of viral RNA from human serum and biopsy samples. J. NIH Res. 6:83. 1994.