BCP – Phase Separation Reagent (BP 151)
Phase Separation Reagent is molecular biology grade 1–bromo–3–chloropropane (BCP). It replaces chloroform, the highly volatile compound commonly used for phase separation in the single-step method of total RNA isolation and in the TRI Reagent® method. Substituting BCP for chloroform moderately improves the quantity and quality of the isolated RNA (1).
- Chomczynski, P. and Mackey, K. Substitution of chloroform by bromochloropropane in the single-step method of RNA isolation. Anal. Biochem. 225: 163-164. 1995.