Bactozol Kit (BA 154)
Bactozol™ is a kit for the isolation of DNA from gram-negative and gram-positive bacteria. The Kit includes Bactozol™ Enzyme Solution and DNAzol®. Bactozol Enzyme Solution is a solution containing activated lysozyme that effectively lyses a broad range of bacterial specimens. It is supplied as a convenient 10X stock solution that maintains enzyme stability at room temperature and precludes the daily preparation of lysozyme stock solutions. After bacterial lysis with Bactozol Enzyme Solution, DNAzol® is used to isolate high quality DNA in a simple and efficient protocol. The Bactozol Enzyme Solution – DNAzol® procedure isolates
both genomic and plasmid DNA from diverse bacterial samples. The Bactozol procedure can accommodate gram-positive bacteria that are more difficult to lyse and often produce lower DNA recovery, such as Staphylococcus and Streptococcus.
Bactozol™ Kit includes 50 ml of DNAzol®, 1.3 ml of 10X Bactozol™ Enzyme Solution and 15 ml of Dilution Buffer. The kit has sufficient reagents to process 125 bacterial pellets derived from 0.5 – 2.0 ml of culture, each containing up to 40 μg of bacterial DNA.
- Chomczynski, P., Mackey, K., Drews, R., and Wilfinger, W. DNAzol®: A reagent for the rapid isolation of genomic DNA. BioTechniques. 22: 550-553. 1997.