DNAzol® is a complete and ready to use reagent for the isolation of genomic or viral DNA from solid and liquid samples of human, animal and plant origin. The DNAzol procedure is based on the use of a novel guanidine – detergent lysing solution that hydrolyzes RNA and promotes the selective precipitation of DNA from the cell lysate (1, 2). This advanced DNA isolation method combines reliability and efficiency with the simplicity of a fast isolation protocol. DNAzol® is a non-toxic solution and the procedure does not require the use of phenols. DNA can be obtained from a large number of samples of small or large volume; 50 ml of DNAzol® is sufficient to process 50 samples, each with 25 – 50 mg of tissue. Genomic DNA isolation can be completed in 10 – 30 minutes with DNA recovery of 70 – 100%. Isolated DNA can be used for Southern analysis, dot blot hybridization, molecular cloning and polymerase chain reaction. There are 3 versions of DNAzol®, each designed for optimal isolation efficiency from various types of samples.
US Patent 5,945,515.
- Chomczynski, P., Mackey, K., Drews, R., and Wilfinger, W. DNAzol®: A reagent for the rapid isolation of genomic DNA. BioTechniques. 22: 550-553. 1997.
- Mackey, K., Williams, P., Seim, S. and Chomczynski, P. The use of DNAzol® for the rapid isolation of genomic DNA for whole blood. Biomed. Products. Supplement: 13-15. 1996.