RNAzol^®RT is the most effective reagent for isolation of total RNA and small RNA from samples of human, animal, plant, bacterial and viral origin. This patent-pending reagent⁽¹⁾ provides higher yield and quality of isolated RNA than previous reagents based on the single-step method. RNAzol RT isolates pure and undegraded RNA that is ready for RT-PCR without DNase treatment.⁽²⁾

• No chloroform-induced phase separation is necessary to obtain pure RNA. Just add water to remove DNA, proteins, polysaccharides and other contaminants.

• The isolation procedure can be completed in less than one hour and is performed at room temperature, including all centrifugation steps.

• RNAzol RT isolates total RNA, with mRNA and small RNA in separate fractions. Small RNA contains RNA < 200 bases, including 10 - 40 base miRNA.

• The isolated RNA is ready for RT-PCR, qRT-PCR, microarrays, poly A+ selection, northern blotting, RNase protection assay and other molecular biology applications.

• Due to the removal of impurities, the RNA pellets are smaller and solubilize more easily than pellet obtained from previous single-step reagents.

• In addition, RNAzol RT allows for the simultaneous isolation of RNA and DNA.

References

1. Chomczynski, P., Reagents and methods for isolation of purified RNA, US and International Patents Pending.
2. Chomczynski, P. and Sacchi, N. Single-step method of RNA isolation by acid guanidinium
    thiocyanatephenol-chloroform extraction. Anal. Biochem. 162: 156-159. 1987.

            

Agilent RNA Nano Chip and Small RNA Chip analysis of rat liver total RNA (A) and
small RNA (B), respectively. High quality total RNA had a RIN value of 9.2 and
28/18 S ratio of 1.6. miRNA region (B, inset) represents about 5% of small RNA.