Click on the links below for product descriptions and detailed protocols.

TRI Reagent® RT     New ! TRI Reagent® RT Blood     New ! TRI Reagent® RT Liquid Samples     New ! Description of TRI Reagent®  Summary of the TRI Reagent® Procedure Picture of Isolated RNA TRI Reagent® Protocol TRI Reagent® BD Protocol TRI Reagent® LS Protocol Oligo (dT)-cellulose columns BCP - Phase Separation Reagent Polyacryl Carrier FORMAzol® Microcarrier™ Microcarrier™ Gel-TR High Salt Precipitation Solution Phosphate Buffer for Spectrophotometry

DESCRIPTION OF TRI REAGENT^®
TRI Reagent^® is a patented reagent for the isolation of total RNA or for the simultaneous isolation of RNA, DNA and proteins. The reagent is an improved version of the popular single-step method for total RNA isolation (1,2,3). It is a monophase solution containing phenol and guanidine thiocyanate. TRI Reagent provides the most reliable, cost effective and efficient method of RNA isolation, and it is the only commercial method available offering simultaneous isolation of RNA, DNA and protein from a single biological sample. TRI Reagent allows for the most comprehensive analysis of gene expression in a variety of samples of human, animal, plant, yeast, bacterial and viral origin. RNA isolation is complete in less than one hour, and DNA and protein isolations in less than three hours. There are three versions of TRI Reagent available, each designed for optimal isolation efficiency from certain types of samples.

TRI Reagent^® (cat. no. TR 118) is used for RNA isolation from tissues, pelleted cells and cells grown in monolayer. Fifty milliliters is sufficient to process 50 samples, each containing 50 – 100 mg of tissue. Expected yields range from 50 – 700 µg of RNA per sample, depending upon the tissue source. 

TRI Reagent^® BD (cat. no. TB 126) is designed for use with whole blood and plasma. Fifty milliliters is sufficient to process 65 samples of 0.25 ml each. 

TRI Reagent^® LS (cat. no. TS 120) is used for cell suspensions and other liquid samples. Fifty milliliters is sufficient to process 65 samples of 0.25 ml each. 

REFERENCES

1. Chomczynski P. and Sacchi N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem.,162,156-159.
2. Chomczynski P. 1993. A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. BioTechniques, 15, 532-537. 
3. Chomczynski P., Bowser-Finn R. and Sabatini L. 1994. A reagent for the single-step isolation of viral RNA from human serum and biopsy samples. J. NIH Res., 6, 83. 

US Patents 4,843,155 and 5,346,994.

PICTURE OF ISOLATED RNA

TRI Reagent^® was used to isolate total RNA
from rat kidney (lane 1), P₀ cells (lane 2),
leafy sprouts (lane 3) and human blood (lane 4).