HIGH SALT PRECIPITATION SOLUTION
Cat. No. PS 161
Store at room temperature.

PRODUCT DESCRIPTION
High Salt Precipitation Solution is a molecular biology grade solution of sodium chloride and sodium citrate specially designed for RNA precipitation in the TRI Reagent®, (cat. no. TR 118) procedure. The procedure involves precipitation of RNA with isopropanol in the presence of High Salt Precipitation Solution. This modified precipitation procedure effectively prevents polysaccaride and proteoglycan contamination of RNA isolated from sources rich in these compounds (1).

APPLICATION
Perform sample homogenization and phase separation according to the TRI Reagent protocol and transfer the aqueous phase containing total RNA to a clean tube. Precipitate RNA from the aqueous phase by the addition of isopropanol followed by High Salt Precipitation Solution. Add 0.25 ml of isopropanol and 0.25 ml of the High Salt Precipitation Solution per 1.0 ml of TRI Reagent used in the initial homogenization. Mix the resulting solution by inversion, store at room temperature for 5 - 15 min and centrifuge at 12,000 g for 8 min at 4 - 20 C. Wash the RNA pellet and solubilize as described in the TRI Reagent protocol.

SPECIFICATIONS
Molecular biology grade, RNase-free.
Contains:   1.2M NaCl and 0.8M C₆H₅Na₃O₇

REFERENCES

1. Chomczynski, P and Mackey, K. 1995. Modification of the TRI Reagent procedure for isolation of RNA from polysaccaride- and proteoglycan-rich sources. BioTechniques 19, 492-495.