POLYACRYL CARRIER PRODUCT DESCRIPTION APPLICATION PolyAcryl Carrier contributes to the
optical density of RNA and DNA preparations. To normalize for this effect, process a blank sample containing only the reagent and PolyAcryl Carrier. Solubilize the final pellet of the carrier in the same volume of solubilization solution used for the test sample
pellets. Measure the optical density at 260 and 280 nm for all samples and the blank, and subtract the values of the blank from values obtained for each DNA sample. MRC GUARANTEE
Cat. No. PC 152
Store at 4 C.
PolyAcryl Carrier is a molecular biology grade solution of acryl polymer designed for use in the isolation of small amounts of RNA or DNA. PolyAcryl Carrier does not affect the
activity of restrictases, reverse transcriptase, Taq polymerase, DNA polymerase, ligase
or other enzymes used for nucleic acid analysis. The carrier can be used for at least one year when stored at room temperature or 4 C. The shelf-life can be extended beyond one
year by storage at -20 C.
For the
isolation of RNA or DNA using TRI Reagent®, DNAzol® or RNAzol® RT
, add 2 - 8 µl of PolyAcryl carrier per 1.0 ml of TRI Reagent, DNAzol or RNAzol® RT.
Perform homogenization or lysis of the sample and isolate the RNA or DNA as described in the
respective protocols. For RNAzol RT, Polyacryl Carrier PC 152 is used for the precipitation
of total RNA or small RNA. For the precipitation of mRNA, please use Precipitation Carrier PC 173
as described in the RNAzol RT protocol. For the precipitation of nucleic acids from other solutions,
add 2-8 µl of PolyAcryl carrier per 1.0 ml of RNA or DNA solution. Briefly mix the solution and
carry out precipitation by adding NaCl to a final concentration of 0.2 M,
followed by 2.5 volumes of ethanol. Mix the solution and store at room temperature for
5-10 minutes. Sediment DNA precipitate at 3,000 g or RNA precipitate at 10,000 g for 10
minutes at 4 C. Dissolve the precipitate by repetitive pipeting in DEPC-treated water or other solubilizing medium.
RNase and DNase activity - none detected.
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Last modified: April 4, 2012