PRODUCT DESCRIPTION
Microcarrier^TM Gel is an ultra pure, RNase-free, DNase-free polysaccharide gel designed specifically for use in the precipitation of small amounts of RNA and DNA. Composed of predominantly high-molecular weight polymer of a-D-glucose, Microcarrier^TM Gel does not affect the activities of enzymes used for analysis of DNA and RNA such as restrictases, reverse transcriptase, Taq polymerase, DNA polymerase or ligase. When stored at 4 C, Microcarrier^TM Gel can be used as a co-precipitant of nucleic acids for at least one year. The shelf-life can be extended beyond one year by storage at -20 C.

APPLICATION
Add 2-10 µl of Microcarrier^TM Gel per 1.0 ml of RNA or DNA solution. Briefly mix the solution and carry out precipitation by adding NaCl to a final concentration of 0.2 M, followed by 2.5 volumes of ethanol. Store the solution at room temperature for 5-10 minutes and sediment the DNA precipitate at 3,000 g and the RNA precipitate at 10,000 g for 10 minutes at 4 C. Dissolve the precipitate by repetitive pipeting in DEPC-treated water or other solubilizing medium.

MRC GUARANTEE
RNase activity - none detected
DNase activity - none detected
sulfate < 0.003%
Ca < 0.0005%
Mg < 0.0005%
Cu < 0.0005%
Pb < 0.0005%

[ Home ][ Up ] Send mail to webmaster@mrcgene.com with questions or comments about this web site. Copyright© 1998-2003 Molecular Research Center Last modified: December 29, 2003