If you have ever used the single-step acid phenol method to isolate RNA, you will love the convenience and reliability of TRI Reagent.

• Developed and patented by Dr. Piotr Chomczynski, founder of Molecular Research Center, the TRI Reagent method is an improved version of his popular single-step method of total RNA isolation. TRI Reagent inhibits RNase more effectively and isolates about 30% to 100% more RNA than the original single step method.


• TRI Reagent allows you to isolate RNA, DNA and proteins from the same sample simultaneously.


• RNA isolation can be completed in less than one hour and DNA and protein isolation in less than three hours.


• TRI Reagent provides the highest quality RNA with yields of: 7-10 ug RNA/mg tissue from pancreas, liver and spleen; 1-2 ug RNA/mg tissue from brain and muscle, and 5-10 ug RNA/10⁶ cultured cells.
• The high quality of total RNA isolated with TRI Reagent assures the best results in RT-PCR, northern blotting, RNase protection, cDNA cloning, differential display and other molecular biology applications.
• Since the introduction of TRI Reagent in 1993, this patented technology has become the method of choice in a variety of applications, providing the most reliable, cost effective and efficient method of total RNA isolation from samples of human, animal, plant, yeast, bacterial and viral origin. TRI Reagent has been used successfully for RNA isolation from a variety of samples including cells on space shuttle missions, samples collected in Thailand's jungles and samples from lobsters collected in Maine's coastal waters.

THERE ARE THREE VERSIONS OF TRI REAGENT AVAILABLE:

To read the complete product protocols, please click on the links below:

• TRI Reagent® (cat. no. TR 118) is used for RNA isolation from tissues, pelleted cells and cells grown in monolayer. Fifty milliliters is sufficient to process 50 samples, each containing 50 – 100 mg of tissue.
• TRI Reagent® BD (cat. no. TB 126) is designed for use with whole blood and plasma. Fifty milliliters is sufficient to process 65 samples of 0.25 ml each.
• TRI Reagent® LS (cat. no. TS 120) is used for cell suspensions and other liquid samples. Fifty milliliters is sufficient to process 65 samples of 0.25 ml each.