Click on the links below for product descriptions and detailed protocols.
DNAzol® is a complete and ready to use reagent for the
isolation of genomic or viral DNA from solid and liquid samples of human, animal
and plant origin. The DNAzol procedure is based on the use of a novel guanidine
- detergent lysing solution that hydrolyzes RNA and promotes the selective
precipitation of DNA from the cell lysate (1, 2). This advanced DNA isolation
method (U.S. patent no. 5,945,515) combines reliability and efficiency with the
simplicity of a fast isolation protocol. DNAzol is a non-toxic solution and the
procedure does not require the use of phenols. DNA can be obtained from a large
number of samples of small or large volume; 50 ml of DNAzol is sufficient to
process 50 samples, each with 25 - 50 mg of tissue (3, 4). Genomic DNA isolation
can be completed in 10 - 30 minutes with DNA recovery of 70 - 100%. Isolated DNA
can be used for Southern analysis, dot blot hybridization, molecular cloning and
polymerase chain reaction. There are 3 versions of DNAzol, each designed for
optimal isolation efficiency from various types of samples.
(cat. no. DN 127) is for use with tissues, cells and liquid samples. 1 ml
of DNAzol isolates DNA from 25 - 50 mg tissue or from cell pellets containing 1
- 3 x 107 cells.
DNAzol® ES (cat. no. DN 128)
is extra strength DNAzol for the isolation of genomic DNA from
1.5 ml of DNAzol ES isolates DNA from 0.5 g of plant
DNAzol® BD (cat. no. DN 129)
is specifically designed for the isolation of genomic or viral DNA from whole
blood. 1.5 ml of DNAzol BD isolates DNA from 0.5 ml of whole blood.
- Chomczynski P., Mackey K., Drews R., Wilfinger W. 1997. DNAzol®: A reagent
for the rapid isolation of genomic DNA. BioTechniques 22, 550-553.
- Mackey K., Williams P., Seim S., Chomczynski P. 1996. The use of DNAzol®
for the rapid isolation of genomic DNA from whole blood. Biomedical Products
- Mackey K., Steinkamp A. and Chomczynski P. 1997. DNA Extraction from Small
Blood Volumes and Single-Tube DNA Extraction and Amplification Using Blood
Filter Cards. Molecular Biotechnology, 9, 1-5.
- Chomczynski P., Wilfinger W. and Mackey K. 1998. Isolation of Genomic DNA
from Human, Animal and Plant Samples with DNAzol Reagents. Biotechnology
THE DNAzol® PROCEDURE
|Examples of expected DNA yields
using DNAzol |
||µg DNA / mg tissue
*yields from plant vary widely depending upon the sample
|4.3 ± 0.7
3.6 ± 0.2
2.0 ± 0.4
2.5 ± 0.4
0.05 - 0.30
35 ± 3.9
PICTURE OF ISOLATED
DNAzol was used to isolate genomic DNA
liver tissue (lane 1-2),
human blood (lanes 3-4) and
adult growth leaves (lanes 5-6).
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Last modified: February 16, 2011