DNA isolation

DNA ISOLATION



Click on the links below for product descriptions and detailed protocols.


DESCRIPTION OF DNAZOL®
DNAzol® is a complete and ready to use reagent for the isolation of genomic or viral DNA from solid and liquid samples of human, animal and plant origin. The DNAzol procedure is based on the use of a novel guanidine - detergent lysing solution that hydrolyzes RNA and promotes the selective precipitation of DNA from the cell lysate (1, 2). This advanced DNA isolation method (U.S. patent no. 5,945,515) combines reliability and efficiency with the simplicity of a fast isolation protocol. DNAzol is a non-toxic solution and the procedure does not require the use of phenols. DNA can be obtained from a large number of samples of small or large volume; 50 ml of DNAzol is sufficient to process 50 samples, each with 25 - 50 mg of tissue (3, 4). Genomic DNA isolation can be completed in 10 - 30 minutes with DNA recovery of 70 - 100%. Isolated DNA can be used for Southern analysis, dot blot hybridization, molecular cloning and polymerase chain reaction. There are 3 versions of DNAzol, each designed for optimal isolation efficiency from various types of samples.

DNAzol® (cat. no. DN 127) is for use with tissues, cells and liquid samples. 1 ml of DNAzol isolates DNA from 25 - 50 mg tissue or from cell pellets containing 1 - 3 x 107 cells.

DNAzol® ES (cat. no. DN 128) is extra strength DNAzol for the isolation of genomic DNA from plants.
1.5 ml of DNAzol ES isolates DNA from 0.5 g of plant tissue.

DNAzol® BD (cat. no. DN 129) is specifically designed for the isolation of genomic or viral DNA from whole blood. 1.5 ml of DNAzol BD isolates DNA from 0.5 ml of whole blood.

REFERENCES

  1. Chomczynski P., Mackey K., Drews R., Wilfinger W. 1997. DNAzol®: A reagent for the rapid isolation of genomic DNA. BioTechniques 22, 550-553.
  2. Mackey K., Williams P., Seim S., Chomczynski P. 1996. The use of DNAzol® for the rapid isolation of genomic DNA from whole blood. Biomedical Products Supplement, 13-15.
  3. Mackey K., Steinkamp A. and Chomczynski P. 1997. DNA Extraction from Small Blood Volumes and Single-Tube DNA Extraction and Amplification Using Blood Filter Cards. Molecular Biotechnology, 9, 1-5.
  4. Chomczynski P., Wilfinger W. and Mackey K. 1998. Isolation of Genomic DNA from Human, Animal and Plant Samples with DNAzol Reagents. Biotechnology International, 185-188.

SUMMARY OF THE DNAzol® PROCEDURE

Summary of the DNAzol Procedure



Examples of expected DNA yields using DNAzol
source µg DNA / mg tissue n
liver 
kidney 
spleen 
heart 
lung 
skeletal muscle 
pituitary cells 
plant tissue* 
blood 

*yields from plant vary widely depending upon the sample material.

 4.3 ± 0.7 
3.6 ± 0.2 
22.8 ± 4.1 
2.0 ± 0.4 
2.5 ± 0.4 
0.8 
6.9 µg/106 cells 
0.05 - 0.30 
35 ± 3.9		7 
12 
5 
3 
3 
1 
1 

6

PICTURE OF ISOLATED DNA

Image of isolated DNA
DNAzol was used to isolate genomic DNA
from rat liver tissue (lane 1-2),
human blood (lanes 3-4) and adult growth leaves (lanes 5-6).

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Last modified: February 16, 2011