BCP - PHASE SEPARATION REAGENT
Cat. No. BP 151
Store at room temperature. This product is stable for at least two years from the date of purchase but should be stored away from heat and ignition sources. BCP vapors are flammable and empty containers should be placed in a fume hood to exhaust any residual combustible material before disposal of the container.
The Phase Separation Reagent consists of molecular biology grade 1-bromo-3-chloropropane (BCP). BCP replaces chloroform in the single-step method of RNA isolation or the simultaneous isolation of RNA, DNA and proteins. Substituting BCP for chloroform does not affect the quality of isolated RNA, DNA or proteins; however the use of BCP as a phase separation reagent may decrease the possibility of contaminating RNA with DNA (P. Chomczynski and K. Mackey, Anal Biochem. 225:163-164,1995).
Phase separation reagent can be used with TRI Reagent« (cat. no. TR 118), TRI Reagent« LS (cat. no. TS 120) and TRI Reagent« BD (cat. no. TB 126). Following sample homogenization, supplement the homogenate with 0.1 ml of BCP per 1.0 ml of TRI Reagent used for the homogenization. This volume of BCP replaces 0.2 ml of chloroform required in the TRI Reagent procedure. Vigorously mix the homogenate with BCP and proceed with centrifugation and other steps of the TRI Reagent protocol. BCP replaces chloroform in all versions of the single-step method and can also replace chloroform in other protocols for the isolation of nucleic acids.
Tested as a phase separation reagent in TRI Reagent protocols for RNA, DNA and protein.
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Last modified: February 16, 2011.