BACTOZOL

BACTOZOL KIT
BACTERIAL DNA ISOLATION KIT


DESCRIPTION OF BACTOZOL ™
Bactozol ™ is a kit for the isolation of DNA from gram-negative and gram-positive bacteria. The Kit includes three reagents: 10X Bactozol Enzyme Solution™, Bactozol Enzyme Dilution Buffer and DNAzol®. Bactozol Enzyme Solution is a novel solution containing activated lysozyme for the most effective lysis of a broad range of bacterial specimens. It is supplied as a convenient 10X stock solution that maintains enzyme stability at room temperature and precludes the daily preparation of lysozyme stock solutions. After bacterial lysis with 1X Bactozol Enzyme Solution, DNA is isolated from the lysate with DNAzol (1). The Bactozol Kit protocol produces high quality bacterial genomic and plasmid DNA with a simple and efficient protocol. The Kit includes sufficient reagents for 125 isolation procedures, each yielding up to 40 µg of DNA.

10X Bactozol Enzyme Solution (cat. no. BZ 160) also may be purchased separately. After lysing bacteria with Bactozol Enzyme Solution, you may apply any DNA or protein isolation method appropriate to your research needs. A sufficient amount of Bactozol Enzyme Solution is provided to prepare 125 lysates, each yielding up to 40 ug of bacterial DNA.

SUMMARY OF THE BACTOZOL PROCEDURE

Summary of the Bactozol Procedure

Approximate yields from bacteria processed using BactozolTM
bacteria µg DNA /109 cells* n
gram-negative 
Escherichia coli 
Citrobacter braaki 
Agrobacterium
rhizogenes
 
gram-positive 
Bacillus subtillis 
Micrococcus luteus 
 
16.3 ± 0.3 
17.5 

38.5 
 
 
 7.3 ± 0.4 
14.0 

*bacterial cell density estimated by absorbance at 600 nm (0.1 OD unit = 1 x 108 bacteria/ml).

  
3 
1 
1 
 
 
3   
1

PICTURE OF ISOLATED DNA

Image of isolated DNA
DNA(ug/lane) isolated from gram-negative
(1) Escherichia coli,
(2) Citrobacter braakii,
(3) Agrobacterium rhizogenes and
gram-positive (4) bacillus subtillis,
(5) Micrococcus luteus.

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Last modified: August 22, 2006