P. Chomczynski and N. Sacchi's original paper, Single Step Method of RNA Isolation by Acid Guanidinium Thiocyanate Phenol Chloroform Extraction (Analytical Biochemistry, vol. 162, p.156) has been cited over 50,000 times since its publication in 1987, as determined by the Institute for Scientific Information (who publish Science Citation Index and Journal Citation Reports). The newest MRC product, RNAzol®RT, is a patent pending improvement on this historical method.
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